What Is Roller Bottles Cell Culture - Fudau Biotech

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Roller bottles are a type of cell culture vessel used in the field of cell biology and biotechnology for the cultivation and expansion of adherent cells. Adherent cells are cells that require a surface to attach and grow, and roller bottles provide a unique environment for their propagation.

Here's an overview of roller bottles in cell culture:

Roller Bottle Design:

Shape: Roller bottles are typically cylindrical in shape with a horizontal axis. They resemble a soda bottle laid on its side.

Material: They are commonly made of polystyrene or other materials that are suitable for cell culture applications.

Surface Treatment: The inner surface of roller bottles is usually treated to promote cell attachment. This treatment may involve coating the surface with substances like collagen or other extracellular matrix components.

Cell Culture Roller Bottle

Operation:

Rotation: Roller bottles are mounted on a roller apparatus that continuously rotates them. The rotation ensures a dynamic environment, exposing cells to nutrients and oxygen as they circulate in the medium.

Media Flow: As the roller bottles rotate, the culture medium inside moves in a wave-like fashion. This movement is critical for providing nutrients to the cells and removing waste products.

Advantages of Roller Bottle Cell Culture:

High Surface Area: Roller bottles offer a large surface area for cell attachment, allowing for the cultivation of a higher number of cells compared to traditional culture flasks.

Low Shear Stress: The rolling motion of the bottles generates low shear stress on the cells, which is beneficial for maintaining the integrity and functionality of certain cell types.

Large-Scale Culture: Roller bottle systems are suitable for large-scale cell culture processes, making them advantageous for industrial production of biological products, such as vaccines or therapeutic proteins.

Uniform Cell Distribution: The rolling motion helps to distribute cells evenly on the culture surface, promoting consistent growth and reducing clumping.

Cell Culture Roller Bottle

Applications:

Vaccine Production: Roller bottles are commonly used in the production of vaccines, where large quantities of cells are required to produce viral vaccines.

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Monoclonal Antibody Production: The cultivation of hybridoma cells for the production of monoclonal antibodies is another application of roller bottle cell culture.

Virology Studies: Roller bottles are utilized for the propagation of certain types of viruses for virology research.

Considerations:

Cell Type Suitability: Roller bottles are particularly well-suited for adherent cell types. Suspension cells, which do not require a surface for attachment, are typically cultured in alternative vessels.

Media and Conditions: The choice of culture medium and environmental conditions, such as temperature and CO2 levels, should be optimized based on the specific requirements of the cells being cultured.

In summary, roller bottle cell culture is a method used for the large-scale cultivation of adherent cells. The rolling motion of the bottles provides a dynamic environment that supports cell growth and is particularly useful in industrial applications for the production of various biological products.

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Cell cell culture roller bottles are generally used in cell culture at the same time as spinner bottles, which can change cell culture from static to dynamic. It is a simplified bioreactor and is suitable for the cultivation of suspension cells and adherent cells. This cell culture method Known as cell culture roller bottles culture technology.

Cell culture roller bottles culture is a cell transition stage before the maturity of large-scale culture technology. It can be used as a way for bioreactor inoculation of cell culture medium preparation . Compared with traditional static monolayer culture, cell culture roller bottles culture technology has three advantages:

1. Provide a larger growth surface for cells;

2. Slight rotation can prevent some components formed in the culture medium from possibly affecting cell growth;

Cell Culture Roller Bottles

3. Cells are only covered with a thin layer of culture medium most of the time, which is conducive to gas exchange;

4. Simple structure, low investment, mature technology and good repeatability;

5. To scale up, simply increase the number of spinner bottles.

In the field of biomedicine, cell culture roller bottles culture technology is mainly used for diploid cell culture to produce measles virus, mumps virus and Japanese encephalitis virus, and for CHO cell culture to produce erythropoietin (EPO). In the field of animal vaccines, it is mainly used to cultivate primary cell CEF to produce Marek's virus (Jiangxi Merial) and chicken pox virus, and also used for rapid and large-scale expansion of seed cells in the early stage of production of swine fever, blue ear disease, circus and pseudorabies virus Increase process.

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The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.

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Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.

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In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.

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