Maximal and minimal volume in T75 and T25 flask

Hi,


I have a question about the maximal and minimal volumes of culture medium to be used in a flask of T75 and T25 ?
Please, anyone knows how many ml at maximum and at minimum should I use in each flask ?


Thank you in advance

If you are looking for more details, kindly visit t225 flasks.

-Biog-

Biog on Feb 18 2009, 08:43 AM said:

Hi,


I have a question about the maximal and minimal volumes of culture medium to be used in a flask of T75 and T25 ?
Please, anyone knows how many ml at maximum and at minimum should I use in each flask ?


Thank you in advance

For maximum - Check this protocol-online page -

For maximum - Check this protocol-online page - Maintenance of cell culture

For T-25 flask - maximum of 10 ml of medium.
For T-75 flask - maximum of 50 ml of medium.
For T-150 flask - maximum of 100 ml of medium.
For T-225 flask - maximum of 200 ml of medium.

-cellcounter-

Thank you very much, "cellcounter" !

All the best

-Biog-

Okay...here is the proper way to do it...
calculation is as follows;
0.2 to 0.5 x cm2

This means that for a T25cm2 flask the volume range is 5-12.5mls for optimal CO2 penetration
T75cm2 flask = 15-37.5mls
T150cm2 flask = 30-75mls
T185cm2 flask = 37-92.5mls

calculation is done by multiplying 25 (for a T25cm2 flask) x 0.2 = 5ml lowest volume
25 x 0.5 = 12.5ml highest volume
75 x 0.2 = 15ml lowest volume
75 x 0.5 = 37.5ml highest volume and so on.

The measurements are gotten by measuring the base of the flask (or bottom). T75cm2 flasks are about 8cm x 9cm. You can even stand the flask up and use it that way. For a T75cm2 flask the bottom measures about 7.5cm x 3cm = 22.5cm2 = 4.5 to 11.2 ml volume. Get it? It really is based off a calculation that is imparitive for CO2 to penetrate the entire volume. Do not go by guessing or by what will and will not spill out of the flask. Hope this helps.

-sciencedork-

sciencedork on Mar 9 2009, 01:02 PM said:

Okay...here is the proper way to do it...
calculation is as follows;
0.2 to 0.5 x cm2

This means that for a T25cm2 flask the volume range is 5-12.5mls for optimal CO2 penetration
T75cm2 flask = 15-37.5mls
T150cm2 flask = 30-75mls
T185cm2 flask = 37-92.5mls

calculation is done by multiplying 25 (for a T25cm2 flask) x 0.2 = 5ml lowest volume
25 x 0.5 = 12.5ml highest volume
75 x 0.2 = 15ml lowest volume
75 x 0.5 = 37.5ml highest volume and so on.

The measurements are gotten by measuring the base of the flask (or bottom). T75cm2 flasks are about 8cm x 9cm. You can even stand the flask up and use it that way. For a T75cm2 flask the bottom measures about 7.5cm x 3cm = 22.5cm2 = 4.5 to 11.2 ml volume. Get it? It really is based off a calculation that is imparitive for CO2 to penetrate the entire volume. Do not go by guessing or by what will and will not spill out of the flask. Hope this helps.

OOOh ! I completely forget this !
Thanks a lot.
<>

-Biog-

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If you want to learn more, please visit our website t75 cell culture flask.

OOOh ! I completely forget this !Thanks a lot.

Parameter of PakGent T225 cell culture flasks

Part No.DescriptionPackageSize/CaseCBM/CaseGW/Case (kg)CL-F225PCell Culture Flask with Plug Cap, PS, 225cm2, TC treated5pcs/pack, 5packs/case78*33*28cm0.07215.9

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PakGent T225 cell culture flasks Features

Design Appearance: The PakGent T225 cell culture flasks with plug caps typically have a rectangular or square shape with rounded corners. 

Capacity: The "T225" designation refers to the approximate flask working volume t225, which is 225 ml. 

Material: The PakGent T225 cell culture flasks are commonly made from high-quality and optically clear polystyrene plastic. 

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T225 Flask Cell Culture Application

Cell Culture Expansion: The T225 flasks are primarily used for the expansion of adherent cells in culture. T225 flask working volume of growth surface area allows cells to spread out and grow in a monolayer.

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Gas Exchange: The T225 flasks feature a vented cap or filter cap, which allows for gas exchange while preventing contamination.

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What is the PakGent t225 cell culture flask volume

The PakGent T225 cell culture flask has a volume of 225 cubic centimeters (225 cm³) or 225 milliliters (225 mL). This generous volume is designed to provide ample space for cell growth and culture, accommodating a wide range of cell types and experimental conditions.

How much media is in a PakGent T225 cell culture flask?

A PakGent T225 cell culture flask typically contains 225 cubic centimeters (cm³) or milliliters (mL) of growth medium when filled to the recommended working volume. The T225 flask is specifically designed to accommodate this volume, providing a spacious environment for cell cultivation. The media volume required for optimal cell culture in a T225 flask may vary based on the specific cell type, growth conditions, and experimental protocols. It is important to ensure that the appropriate amount of growth medium is added to the flask to support the growth, proliferation, and viability of the cultured cells. Adhering to the recommended media volume guidelines helps to maintain optimal nutrient availability, pH balance, and osmotic conditions within the flask, contributing to successful cell culture outcomes and experimental reproducibility.

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